Diabetes is becoming more widespread over the world. Egypt is among the top ten countries in the world in the number of diabetes patients according to the International Diabetes Federation. Bisphenol A is widely used in the production of polycarbonate plastics, epoxy resins, food and beverage containers. Bisphenol A is a metabolic disruptor in addition to an endocrine disruptor. Purpose: This study aims to evaluate the possible association between bisphenol A and type 2 Diabetes Mellitus among Egyptian samples. Methods: We collected urine samples from (100) adult participants dividing into two major groups, cases group consisted of 50 patients with type 2 DM and control group consisted of 50 normal adult persons. Subjects in the cases group were selected from the attendants of the Diabetes Endocrine & Metabolism outpatient clinic, Kasr-Alainy, Cairo University hospitals. In urine, bisphenol A was measured using high-performance liquid chromatography (HPLC). Results: Regarding bisphenol A levels among the studied groups, there was a highly significant statistical difference between the cases and the control group with p value<0.001. The mean body mass index was 27.32 ± 3.32 for cases and 25.02 ± 1.87 for the control group with p value<0.001.