Background: Nurses are the largest human resources in hospitals andhave an important role in maintaining and improving the quality of health services in hospitals. The behavior of nurses in professional development is important to study because nurses are an integral part in patient care and play an important role in improving the quality of hospitals, Aim: The aim of this study is to assess the relationship between factors affecting career development and nurses' motivation. Study design was ‎ descriptive correlational design. Sample: a‎ convenience study sampling from available nursing staff. Setting: The critical care units located at Beni-Suef model hospital, Tool I: factors affecting career development questionnaire and Tool II: actors affecting the nurses' work motivation questionnaire. Results: The present study indicated that majority of the studied group had satisfactory level regarding factors affecting both career development and work motivation with the percentage of (75.5% & 77.3%) respectively. The conclusion: There was highly statistically positive correlation between total factors affecting both career development and work motivation among the studied nursing staff.. The recommendation: Generalize development of policies and plans for enhancing career development and nurses' motivation. In addition, Development of a rewards system to enhance nurse's motivation for improvement and achievements.