Objective: Early Childhood Caries (ECC) is one of the major health problems. Understanding how parental psychological status affects children's oral health is important. Therefore, the aim of this study was to investigate the relationship between parental depression, anxiety and stress with ECC. Methods: In this cross-sectional study, children referring to the Shahrekord public and private dental centers in 2020 were included. Depression and anxiety of children's parents were determined by Beck questionnaire and their stress was determined by parenting stress questionnaire. Background information including gender, children's eating habits, number of children in the family and the reason for the children's first visit to the dentist were also obtained by the checklist. Pediatric DMFT and ECC index were determined through clinical examination and children were divided into two groups with and without ECC (88 and 44 patients, respectively). The analysis was performed by SPSS21 software. Results: There was no significant difference between the two groups in terms of gender, father's level of education and parents' occupation, but there was a significant difference in terms of mother's level of education, sugar consumption and age (respectively P=0/01, P=0/002 and P >0/001). The mean score of parental depression in the group with ECC was significantly higher than the group without ECC (P=0/003) but stress and anxiety were not significantly different between the two groups. The mean scores of depression, stress and anxiety of parents in the two groups with and without ECC were not significantly different according to the sex of children. In additional, slight inverse linear correlations were observed between DMFT index and variables of parental depression and anxiety of children with ECC, but none of them were statistically significant. Conclusion: The results of the present study showed the relationship between ECC and maternal's level of education, child’s sugar consumption and parental depression