Background: Conventional tissue processing of histologic specimens has been carried out for many years, which is a time-consuming method resulting in 1-day delay in diagnosis. The time honored conventional method of tissue processing, is now seeing the trend of being replaced, owing to fulfill the needs of clinicians for rapid diagnosis. The most commonly employed means of tissue processing are conventional tissue processing, rapid tissue processing, microwave tissue processing and automatic tissue processing. Aim: The present study aims to compare the different tissue processing techniques- conventional, rapid conventional, microwave and automatic tissue processor in terms of the effect on staining, cytoplasmic-nuclear details and tissue shrinkage. The time factor involved in the various processes is also assessed. Methodology: A total of 40 biopsied tissue samples were included in the study. The specimens were fixed in 10% formalin for 24 hours to ensure adequate fixation. The specimens were then subsequently photographed and cut into four equal halves to be processed by conventional, rapid conventional, microwave and automatic tissue processor. The sections obtained by the above processing techniques were stained by haematoxylin and eosin stain, and were subjected to a blind study. Each slide was evaluated by four independent observers and were graded into excellent, good, average and poor, based on the cytoplasmic and nuclear morphology and texture of the tissue of the stained sections.