Background : Diabetic Mellitus is one of the common illness caused by metabolic disorders mainly by defect in blood glucose level. It looked at diabetes as two different diseases: type I and type II. In the younger onset group, which consists of people whose age at diabetes diagnosis was less than thirty years, retinopathy was noted in 13% of patients with less than five years of diabetes and 90% of patients with ten to fifteen years of diabetes. Diabetic Retinopathy (DR) affects 25% of those with type 1 diabetes who have had the condition for 15 years. Retinopathy affects 40% of insulin-using patients and 24% of non-insulin-using patients in the older onset group, which consists of people who were diagnosed with diabetes at a period when they were ≥30 and ≤5 years old. Aim : To assess the effect of retinopathy among age & gender of the definite population. Materials and Methods : 200 subjects suffered from DR were included coming in the hospital and done our methodology by using all the parameters after prior consent. Result : It was reported that the most affecting people were of moderate age (51-60 years) having the frequency of 97 and the least affected population were the age of <40 years which were 06. Conclusion : This study reveals that the proper care is needed on the basis of age and gender after examining via different biochemical parameters.