In current Indian scenario, there are plenty of challenges in integrated evidence based nursing practice. Nursing community have basic knowledge and understanding of research and theory but no practical experience of best utilization of research, generating evidence, developing standard research protocol, identification of best search engine and search filters for collection of database, or doing critical appraisals etc. OBJECTIVES: the objective of study was to evaluate the 'Evidence Based Nursing Practices' on Thrombolytic Therapy. Method: Observational research design, was used to evaluate the 'Evidence Based Nursing Practices' on Thrombolytic Therapy in Tertiary Care Hospital, Karad. Analysis was done using the software INSTAT. Results: Observational mean score and SD in ‘evidence based nursing practices’ were 15.18182 and 4.844162 respectively. ‘Evidence Based Nursing Practices’ on thrombolytic therapy scores on nurses shows that majority of samples 72.72% were having good. Conclusion: Based on the finding the result of the study shows 'evidence based nursing practices' on thrombolytic therapy on nursing staff, ‘Evidence Based Nursing Practices’ on thrombolytic therapy scores on nurses shows that majority of samples 72.72% were having good.