Carbapenem-resistant Acinetobacter baumannii (CRAB) is an important health problem for the treatment of infectious diseases. MDR Gram-negative bacteria infections have traditionally been treated with carbapenems as a last option. A. baumannii has lately shown an increase in carbapenem resistance. Enzymatic degradation by -lactamases accounts for the majority of A. baumannii's carbapenem resistance mechanisms. Aim And Objectives: To study the Molecular Characterization of Invasive Carbapenem-Resistant Acinetobacter baumannii with special reference to OXA-51 and OXA-23 gene in ICU patients at a Tertiary care centre. Material And Methods: This was a Cross sectional study carried out in the Department of Microbiology and the Anesthesia Department for a period of 1 year i.e, April 2022 to April 2023 at a tertiary care centre, Kanpur. A total of 110 non-duplicate, consecutive, carbapenem-resistant isolates recovered from Acinetobacter species were included in this study. The isolates were obtained from the clinical samples. The isolates were identified by the standard biochemical tests and the Antimicrobial susceptibility testing was performed according to the CLSI guidelines 2022. Results: In the present study a total of 834 clinical samples were collected in which 110 Acinetobacter species were isolated. The maximum number of isolates were from the ETA samples with 82 (74.5%), 27 (24.5%) from blood and 1 (0.9%) from the tissue. The ratio of Males 76 (69%) was more as compared to that of the Females 34( 30.9%) with the maximum age of 31-40 being affected the most followed by 41-50 and least in the age group above 61 years of age. Antimicrobial susceptibility testing revealed that all the isolates were resistant to ceftazidime, cefepime, piperacillin/tazobactam, cefoperazone/sulbactam, aztreonam, imipenem, meropenem, amikacin, netilmycin, tetracycline, tobramycin, levofloxacin and co-trimoxazole. In the present study it was also observed that around 108 (98.1%) of the study isolates were susceptible to polymyxin B ( colistin). The Molecular characterization reveals that among the 110 isolates tested for MBL, the seven isolates were positive for the blaVIM gene (6.3%) and blaOXA-23 gene was present in all the 110 isolates (100%). Conclusion: Further studies are necessary to monitor the spread of carbapenem-resistant OXA-type lactamase genes from A. baumannii in hospital settings since they are becoming a significant cause of carbapenem resistance. There should be efficient infection control procedures and strict regulation on the use of Antibiotics followed.