Background: The increasing use of toxic pesticides is a major environmental concern. Carbendazim (CBZ) is a systemic fungicide that is considered a persistent environmental contaminant. Linalool is known to reveal various pharmacological activities such as antimicrobial, anti-inflammatory, antioxidant, and anticancer properties. Objectives: The present study investigated the protective mechanism of linalool in CBZ induced thyroid gland damage in rats. Subjects and methods: This experimental study consisted of negative control, positive control, linalool (50 mg/kg), CBZ (500 mg/kg), and combined CBZ and linalool groups for 8 consecutive weeks. 35 rats were randomly divided into five equal groups, G (I): Negative control group: Regular diet and tap water. G (II): Positive control group: 1 ml corn oil (as a vehicle) once daily by oral gavage for 8 weeks. G (III): Linalool treated group: linalool 50 mg/kg B.W. once daily by oral gavage for 8 weeks. G (IV): Carbendazim treated group: 500 mg/kg B.W. of carbendazim (1/10 LD50) dissolved in 1ml corn oil once daily by oral gavage for 8 weeks (Oral LD50 of carbendazim in rats equal 5000 mg/kg). G (V): Carbendazim and linalool-treated group linalool 50 mg/kg then after 1 hour received carbendazim 500 mg/kg once daily by oral gavage for 8 weeks. Serum smples used for evaluating thyroid hormones (TSH, FT3, FT4). Thyroid tissues was used to prepare tissue homogenates, which were used for estimating oxidative stress biomarkers [malondialdehyde (MDA), and superoxide dismutase (SOD)], and pro-inflammatory cytokines [interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α)]. Thyroid tissues blocks were prepared for histopathological examination and immunohistochemistry (IHC) staining using anti-NF-κB and anti-TLR4 antibodies. Results: linalool alleviated CBZ induced a decrease in SOD levels and an increase in MDA levels in thyroid tissues. These biochemical results were supported by histopathological changes which were improved by co-treatment with linalool. Immunohistochemical examination revealed that linalool significantly suppressed CBZ-mediated increase in NF-κB and TLR-4 in the thyroid tissues. Conclusion: linalool-mediated thyroid protection in CBZ-treated rats involves antioxidant and anti-inflammatory.