Polycystic ovary syndrome (PCOS) is a hormonal disorder common among women of reproductive age. Recently use of herbs for the management of polycystic ovary syndrome is interesting. Flaxseed is a rich plant-derived αlinolenic acid of omega-3 polyunsaturated fatty acids, has been proven to benefit for PCOS. Also Licorice is one of the natural antioxidants used for the treatment of infertility. The aim of the present study was to evaluate the effectiveness of licorice and flaxseed in the treatment of Letrozole-Induced PCOS. Twenty-eight female rats were divided into four groups (n = 7/each): (group I): negative control group fed on basal diet. Group II, III and IV were administrated on LTZ 1mg/kg/day orally by gastric gavage for 3 Weeks. Group II kept as positive group (PCOS group) and fed on the basal diet only. While, the remaining other two groups were fed on supplemented diet with 1.5% of flaxseed and licorice, respectively. The obtained results revealed that the groups of rats were fed on flaxseed and licorice had significant increased (p˂0.05) in Follicle-stimulating hormone (FSH), Progesterone and Estradiol (E2) and had significant decreased (p˂0.05) in Insulin, Glucose, luteinizing hormone (LH), Total testosterone, Dehydroepiandrosterone sulfate (DHEA-S), AST, ALT, and Alkaline Phosphate (ALP), compared to the positive group of rats (PCOS rats). Microscopically, results showed improvement in Graffian follicle and corpus luteum in the groups of PCOS rats and were fed on diet supplemented with 1.5% of flaxseed and licorice, respectively as compared to the positive control group. Finally, the existing study illustrated that licorice and flaxseed could improve PCOS syndrome via affecting the serum levels of different hormones and morphology