Background: The rational use of antibiotics in pediatric populations is critical, especially amidst rising concerns over antibiotic resistance. This study analysed antibiotic prescription patterns, administration routes, therapeutic outcomes, and antibiotic sensitivity in a pediatric hospital setting. Methods: In this prospective observational study, data from 300 pediatric patients were analysed. The study included patients ranging from 28 days to 18 years, with exclusions for neonates and those in intensive care units. Antibiotic prescriptions were evaluated for type, administration route, and therapeutic outcomes. Sensitivity patterns against common pathogens like Klebsiella pneumonia, E. coli, Enterococcus faecalis, and Staphylococcus Aureus were assessed. Results: The study found a higher prevalence of antibiotic prescriptions in male patients (59%) and most commonly in the 13-16 years age group. Cephalosporins were the most frequently prescribed antibiotics (53.67%), with parenteral administration being the most common route (53.66%). The majority of patients (59.66%) were cured following antibiotic treatment. Sensitivity analysis revealed the specific effectiveness of certain antibiotics against prevalent bacterial strains. Conclusion: The study highlights the current trends in antibiotic prescription and sensitivity patterns in a pediatric hospital setting. The findings underscore the need for ongoing surveillance and judicious antibiotic use to combat the rise of antibiotic resistance, with implications for pediatric treatment protocols and antibiotic stewardship programs.