The study was conducted to determine the phytochemical content and antibacterial property of Gotu Kola (C. astatica) in two different habitats. Specifically the study sought to answer the following questions: Is there a difference in the phytochemical contents of C. asiatica grown in dry and watery area?: Is there a difference in the antibacterial property of C. asiatica grown in dry and watery area?: Which solvent is effective in extracting the antibacterial component of C. asiatica grown in dry and watery area? The active components of the leaves were extructed using four solvents namely: 95%, 80%, 70% ethyl alcohol and distilled water. The 80% ethyl alcohol was used in the phytochemical screening and the rest were used in the antibacterial assay and were tested against gram-positive bacteria, S. aureus and gram- negative bacteria, E. coli. The investigation of the antimicrobial potential of the extract was carried out through microbiological assay by employing the disk-diffusion method adopted from Kirby-Bauer Technique. The phytochemical screening revealed that C. asiatica contained alkaloids, saponins, flavonoids, hydrolysable tannins and leucoanthocyanins and does not contain anthraquinone. The result of the antibacterial assay showed that all solvents used in the extraction of the antibacterial components of C. asiatica inhibited the growth of S. aureus and E.coli. However, only the pure extracts of each solvent showed a wider clear zone of inhibition with S. aureus. The result further showed that distilled water was the most effective solvent in extracting the antibacterial component of C. asiatica when tested against E.coli. Statistically, the results showed that there was no significant difference in the habitat both for the phytochemical content and antibacterial property.