Monkeypox virus, a DNA virus belonging to the Poxviridae family, is an enveloped virus. It spreads from rodents to primates through infected body fluids, skin lesions, and respiratory droplets. Symptoms of monkeypox virus infection include fever, myalgia, maculopapular rash, and fluid-filled blisters. Distinguishing monkeypox virus from other poxviruses is crucial for accurate diagnosis, which can be achieved through DNA analysis of swab samples. Currently, there is no specific therapy or vaccine available for monkeypox virus, and treatments for other orthopoxvirus infections are used during small outbreaks. In this study, two potential peptides were designed.