UV/VIS ingestion spectrophotometers are complex lab estimation frameworks for substance examination also, testing whose precision and likeness of insightful information can be antagonistically affected by a few significant instrumental boundaries. Reference material normalization, the fundamental idea of insightful estimation science, is the commonsense research facility means to explore potential instrument-related deliberate blunders and, if present, to assess their effect on the quality and dependability of the information. The logical solidness of the approval of an UV/VIS spectrophotometer, whether led in-house or out-obtained, can be no greater than the precision of the reference material principles and the importance of the test technique or on the other hand convention utilized for the approval. Reference material normalization should be the logical foundation to UV/VIS scientific methodology and approval/capability conventions assuming that quality control benefits and administrative consistence are to be accomplished in a convenient and practical way.