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ISSN 2063-5346
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Validation and monitoring of chlorantraniliprole residues in polished and parboiled rice using LC-MS/MS

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Ashok Kumar Karedla1, S.V. Krishnamoorthy1*, A. Suganthi1, K. Bhuvaneswari1, S. Karthikeyan2, P. Geetha2, R. Surya Raj1 P. Karthik1 and J. Kousika1
» doi: 10.48047/ecb/2023.12.10.076

Abstract

Effective analytical method was developed and validated for the detection and quantification of chlorantraniliprole insecticide residues in polished and parboiled rice samples using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and liquid chromatography mass spectroscopy/mass spectroscopy (LC-MS/MS). The method was validated by linearity, limits of detection, limit of quantification, precision and accuracy. At concentrations between 0.01 and 0.1 μg g-1, chlorantraniliprole exhibited good linearity in the matrix, with a correlation coefficient (R2) > 0.98 and a slope ratio showing a matrix attenuation effect. The limits of detection and limits of quantification were 0.004 μg g-1 and 0.012 μg g-1 for polished and parboiled rice. The recovery study was conducted at five different fortification levels ranging from 0.01 to 0.1 μg mL-1. Chlorantraniliprole recovery rates varied from 80.91 to 106.84 and 91.17 to 99.99 percent for polished and parboiled rice, respectively. The current method was found to be quick, easy, accurate, and reliable, making it the perfect analytical strategy for finding residues of chlorantraniliprole in rice samples. Monitoring of super market samples revealed that, out of 30 samples of polished and parboiled rice, none of the samples were contaminated with chlorantraniliprole residues.

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