Background: Indoor air pollutants are constituted by organic compounds, polyaromatic hydrocarbons, transition metals like nickel, chromium etc from biomass smoke, which alter the pulmonary ventilation. Explaining the effect of metal function on pulmonary among cooking women is very minimal. We evaluated the effect of nickel on pulmonary functions among biomass fuel users in rural areas. Methodology: 200 healthy subjects selected biomass fuel use and LPG fuel use from the rural area of Kancheepuram District in India. Written consent and demographic data were collected from every participant before the performance of the test. Pulmonary function tests were done by a computerised Spirometer to assess the FEF25-75% and PEFR% parameters. Serum Nickel was estimated in blood by ICP-MS technology. We used multivariate statistical analysis to find the variation on pulmonary function due to the effect of nickel among biomass fuel users.