Walnut (Juglans Regia L.) is a nut belonging to the family Juglandaceae. In India, it is commonly known as 'Akhrot'. Walnut roots and leaves are traditionally used to treat diabetes, rheumatic pains, fever, and skin diseases; apart from it also contain several biological reported activities in diseases including cardiovascular dysfunction, cancer metabolic syndrome, and various inflammation-related pathological conditions. Objective: The purpose of this review is to provide important insight into the walnut kernel's nutritional importance, traditional uses, and pharmacological potential in life-threatening diseases and to identify the gap in scientific and potential analytical opportunities for future directions on these herbs. Method: Related informative data of this review was collected from renowned online databases namely: Science Direct, Research Gate, Scopus, PubMed, and Scientific Information Database. Results: Omega-3 fatty acids, alpha-linolenic acid, polyphenols, flavonoids, minerals, tannins, monoterpenes, saponins, alkaloids, terpenoids, dietary fibers, and so on were identified from different parts. Moreover, a variety of traditional declarations suggested the pharmacological utilities, and scientific confirmation of its ethnopharmacological characteristics has been well acknowledged. Reported studies suggest the plant has antidiabetic, antioxidant, antimicrobial, anticancer, anti-hypercholesterolemia, and antihypertensive properties. Conclusion This paper draws attention to the use of walnut kernels in a wide array of diseases and provides scientific validation. The presence of active constituents has the potential to cure various medical conditions. Furthermore, it is suggested that the plant brings promising results in reducing the risk of hyperlipidemia, diabetes mellitus, hypertension, cancer, neurodegenerative, and cardiovascular diseases. Therefore, it can be suggested that more emphasis may be laid on clinical trials to be conducted to identify molecules, information pathways, and related genes.