Introduction: Dental enamel is the essential source of observing normal development or abnormal pathological conditions related to past living circumstances faced by human population. Ameloblasts start laying down the most rigid structure, called enamel, subdividing into secretory and maturation stages from intra-uterine life for deciduous teeth. In the literature, special attention is given to one of the incremental lines called a neonatal line which is distinctively observed on all primary teeth at the time of labor. Methodology: A review of literature having a sample size above 100 was done from the year 2000 to 2022 through electronic search engines like PubMed and Google Scholar with the following keywords neonatal line, enamel tooth, primitive teeth diseases, hypocalcification, and gestation period along with thickness and thinness of neonatal lines. Results: We found alterations in the width of the neonatal line with the mean value of 12.4,11.52, and 12.48μm in relation to normal, cesarean, and emergency operative deliveries, respectively. Conclusion: The review shows variation in neonatal line width is associated with multiple factors like mode of childbirth during delivery, complication at birth, birthweight of a newborn, the length or period of gestation, metabolic diseases, and infections, to name a few and this may lead to clinical malformations of teeth or making the teeth more prone to dental caries.