Background: workplace well-being is most importantly proved best working environment to the employees to handle the issues of burnout and poor work engagement, moreover best working condition like supervisor support and motivated environment can enhance the motivation and sense of accomplishment among employees. Objective: The assessment of work life conflict and Relationship between job characteristics and workplace well-being. Method: The correlational research study design was used. The setting of the study was public hospitals of the Lahore. The study target population was Nurses of the public hospitals convenient sampling technique was used. The sample size for this study was (171) calculated through Slovenes formula. Data analysis was analyzed by SPSS version 21. Results: The study's findings indicate that the relationship between work-life conflict and job engagement is positive which is .484 and significant (p value less than.05. Similarly, work-life conflict is positively and strongly related to job demand.459, P. 0.00 Work-life conflict is favorably and strongly related to supervisor support, with SS values of.133 and P.0. Similarly, WLC is significant and positively correlated with the BO.615 and P.0, supporting the study's premise. Similarly, the connection between JD and SS was positive and significant, with P.05 and t 6.321. Conclusion: The results support the hypothesis and explain the connection of employee job characteristics and their workplace well-being. The current study supports the opinion that the work life conflict play a vital role in employee job characteristics and WE. So, therefore, job characteristics play a vital role in employee workplace well-being and when it combines with work to life conflict it gives the negative interference in employee well-being.