The colorectal cancer has been second leading cause of cancer that affects both men and women globally. It indicates approximately 10% of malignant growth –related mortality in Western countries The present study involves wound healing assay and cell viability assay of ethanolic leaf extract of blumea balsamifera against human colorectal carcinoma cell line. The leaf part of Blumea balsamifera was subjected to maceration process using ethanol solvent. The collected crude extract was analysed for Qualitative and quantitative phytochemical analysis, Cell Viability Assay and Wound healing assay. The presence of phytochemicals such as alkaloids, flavonoids, and phenols were identified by qualitative phytochemical screening. Quantitative phytochemical analysis of leaf extract revealed phenol of approximately 141 mg/g, alkaloids of approximately 105 mg/g of atropine equivalents, and flavonoids of approximately 120mg/g of quercetin equivalents. The In vitro Cytotoxicity studies of ethanolic leaf extract of Blumea balsamifera showed IC50 value was found to be 208.25 µg/ml. The in-vitro wound healing activity studies signified with the migration of cells play a vital role in wound repair. It was evidenced that the Blumea balsamifera extract showed 60.18% and 90.89% cell migration observed at 24 h and 48 h, respectively