The findings of our study reveal that zinc administration has a significant impact on the activity of key antioxidant enzymes in Sorghum bicolor. Superoxide dismutase (SOD) activity increased with higher zinc concentrations during the initial 15 days of treatment, reaching a maximum rise of 316% (4-fold) compared to the control with 9.5 mM zinc. However, catalase (CAT) activity decreased in zinc-treated plants, both in 30-day-old and 50-day-old plants. Ascorbate peroxidase (APx) activity showed a complex response, with an initial rise at lower zinc concentrations and subsequent decline at higher concentrations. Glutathione peroxidase (GPx) activity exhibited an age-dependent and concentration-dependent pattern, gradually increasing in 30-day-old plants and showing sustained elevation at 3.5 mM zinc in 50-day-old plants. These findings highlight the intricate interactions between zinc and antioxidant enzymes, providing insights into the modulation of the antioxidant defense system in Sorghum bicolor.