With the continuous use of nano-metal oxides in the field of medicine, health care, and research, metal nanoparticles are developed enormously through various analytical methods which are affordable, researchoriented, and eco-friendly. In the current study, a simple and very useful approach has been followed for the preparation of Zinc oxide nanoparticles with the help of sol-gel method at 370C. The particles synthesized were analysed by X-ray diffraction, electron scanning microscopy, and UV-visible analysis. The temperature 370C, pH level (11.5-11.7) were maintained for the fabrication and the study of the nanoparticles. The λmax value (360 nm) was found to be the most efficient value range in which ultraviolet B is being absorbed. The nanoparticles were then analysed for their antimicrobial activity against highly infectious microbes viz, Bacillus subtilis, Carsonella ruddii, Pseudomonas syringae, Epulopiscium spp, Salmonella typhimurium, Escherichia coli, Myxococcus Xanthus, Bacillus anthracis. The results of antimicrobial activity clearly show that the ZnO-NPs are good antibiotics against these mentioned contagious microbes. So these nanoparticles of zinc could be used as antimicrobial drugs in various forms for different treatments.