Background: Periodontitis is a multifactorial disease resulting in inflammation within supporting tissue of the teeth, progressive attachment and bone loss. The aim of the present study was to assess the efficacy and compare the SYBOGRAFⓇPlus with PRF and PRF alone graft in the treatment of intra-bony defects in chronic periodontitis. Materials and Methods: A total 20 intra-bony defects were selected from patients with chronic periodontitis and divided into three groupsGroup I Open flap debridememt with SYBOGRAFⓇ Plus with PRF and Group II Open flap debridememt with PRF alone. Before taking the clinical parameters, all the patients underwent oral prophylaxis and then recordings were taken at baseline, 3 months and 6 months for Plaque Index, Gingival Index, Pocket Probing depth, Relative Attachment Level and Bone Fill radiographically. Results: The results of the present study showed significant difference in both the groups in inter-group and intra-group analysis except in GI from baseline to 6 months. Conclusion: The overall efficacy in the treatment of chronic periodontitis in terms of clinical parameters (Plaque Index, Gingival Index, Pocket Probing Depth and Relative Attachment Level) and Radiographic parameter (Bone Fill) were shown by Group I (SYBOGRAFⓇPlus with PRF) more significant as compared to Group II (PRF alone).