Every mother’s eagerly waiting the pregnancy and its enjoyment, if pregnancy become Spontaneous the outcome is also good. Due to infertility problem the couple adopting an Assisted Reproductive Technique (ART) method will get adverse outcomes. Aims to compare the perinatal outcome among primi-mothers who conceived spontaneously with IUI/IVF treatment conception. A quantitative research approach & descriptive research design was adopted and conducted at New Life Hospital, Cuddalore. The Convenience Sampling technique was used to select 120 mothers among these 60 mothers were conceived spontaneously conception and 60 mothers conceived IUI/IVF conception. Tools helped to collect the Background variables and the Observation scale was used to assess the perinatal outcome. Results shows that good level of maternal outcome deserved 46 mothers of spontaneous conception mothers but 35 mothers in IUI/IVF and Neonatal outcome observed 48 mothers of spontaneous conception neonates & 38 mothers (63.33%) in IUI/IVF conception neonates. The spontaneous conception group had a mean value = 3.62 and SD = 1.30 and the IUI/IVF conception group had a mean value =5.02 and SD = 1.68 and Mean Difference = 1.40 and “t” value was 5.10 and P=0.001 highly significant. The association was found only with Age in years, occupational status & residence of the mothers in the Spontaneous conception group and IUI/IVF group the association was found in the occupational status, monthly income, and residence of the mother. Study Concludes that Healthy life practices will promote natural conception thereby prevent the poor maternal and neonatal outcomes.