The Revised National Tuberculosis Control Program (RNTCP) in India is currently implementing a standardized treatment regimen (STR) to effectively manage cases of MDR-TB. This study aimed to analyse the results of MDR-TB patients who received treatment with RNTCP's recommended 2-year STR at King George Medical University, Lucknow, in real-life situations. Eligible patients who had previously gone through treatment failure with the category II re-treatment regimen and further on they were tested positive with MDR-TB. From September 2021 to February 2023, 881 patients were choose to participated in the present research work. The research work followed a cross-sectional approach and focused on TB patients who were part of the DOTS program under RNTCP in Lucknow. Purposive sampling was used to select appropriate study locations, with a specific emphasis on Designated Microscopy Centres (DMCs) in the Lucknow district. The research analyzed the data from a total of 881 TB patients, and the overall outcomes for this relatively a less quantity of MDR-TB patients were happens to be treated by following RNTCP's STR in this setting showed promise. It is crucial to underscore the significance of treatment adherence and promptly addressing adverse drug reactions (ADRs) throughout the treatment process.