Chitosan is the second most abundant natural polycationic linear polysaccharide next to cellulose derived from crustacean chitin shells. Chitosan has a variety of commercial and potential biomedical applications. Chitosan nanoparticles (CSNPs) have recently gained popularity due to their bio-nature, biocompatibility, biodegradability, quantum size effects, large surface-tovolume ratios, and ease and low cost of production. This study evaluated the potentiality of Ocimum tenuiflorum (OT) leaf extract-loaded CSNPs as an effective alternative for targeted drug delivery. Tulsi has powerful anti-inflammatory, analgesic, anti-bacterial, and anti-viral properties. Even today, people consume tulsi, decoction, and juice to treat a variety of ailments. When compared to indomethacin, fresh tulsi leaves (TL) in paste form exhibit significant antiinflammatory activity with minimal side effects.