The present study was conducted to find out aquatic weeds in the ponds of Kanyakumari district, Tamilnadu, India during the year 2020 to 2021. A total of 101 plant species belonging to 37 families and 49 genera were documented. Out of 101 species, about 46 Emergent Weeds followed by Submerged weeds (14), Free floating weeds (22) Rooted floating weeds (12), Temporary water situation weeds (7 species). The dominant aquatic weeds were Eichhornia crassipes, Ipomoea carnea, Pistia stratiotes, and Salvinia molesta. The invasive aquatic weeds were Eichhornia crassipes, Ipomoea carnea, Pistia stratiotes, Trapa natans, Typha angustifolia. The medicinal uses of aquatic weeds were Eichhornia crassipes, Hydrilla verticillata, Ipomoea aquatica, Nelumbo nucifera, Typha angustifolia. A number of weeds such as Nelumpo nucifera, Ipomoea aquatica, Trapa natans, Typha angustifolia are reported to have been used for edible use. Various recommendations and measures have been suggested to improve the biodiversity of aquatic weeds of the study areas.