Celiac disease (CD) is a chronic inflammatory disease of the upper small intestine triggered by gluten protein intolerance, which is prevalent in genetically predisposed individuals. The present study aimed to evaluate the beneficial effect of Chia and Quinoa-fortified gluten-free biscuit products on CD in Rats. Thirty-five male Sprague Dawley rats (225±5 g b.wt. and 8 weeks age) were used. Rats were distributed into 5 equal groups; Group1: negative control, group 2: positive control with CD and groups 3, 4 and 5: with CD and were fed Chia, Quinoa and their combination, respectively. Feed intake (FI), body weight gain (BWG %), and feed efficiency ratio (FER) were calculated. Rats were then anesthetized and blood samples were collected for determination of activities of serum liver and kidney function, total cholesterol (TC) and triglyceride (TG) and lipoprotein fractions were measured. Reduced glutathione (GSH), malondialdehyde (MDA) and lactate dehydrogenase (LDH) were measured. Histopathology of small intestine was also carried out. Results revealed that feeding Chia, Quinoa and their combination decreased FI, BWG% and FER, TC and TG and decreased serum parameters of elevated hepatorenal function as compared to the positive control (+ve) group.Histopathological examination of small intestine demonstrated that feeding combination of Chia and Quinoa ameliorated lesions of CD and inflammation. It could be concluded that Chia and Quinoa would be an effective curative for celiac disease- induced histopathological changes in the small intestinedue to its improvement of intestinal histopathological lesions.