Today antimicrobial antibiotic resistance has become main concern for the physicians, healthcare professionals as reported by World Health Organization. The guidelines have been prescribed for the correct usage of the antibiotics or drugs but still the frequency of the resistance is increasing and hospitals are playing major role. The present study was carried out with an objective of to determine rational antibiotics prescription as per guidelines and to visualize the drugs per counter are as per guidelines. The study was conducted in the hospital in Tripura on 250 patients admitted in medicine department. The information was noted from the prescription of the patients admitted in the hospital in pharmacology department of the medicine. The data was written related to average number of drugs per encounter including AMAs and AMAs as per NELM along with information of FDC. For the statistical analysis data was added in the Excel sheet. The different statistical tools were employed to calculate various frequencies, variance, Regression, ANOVA analysis etc. The demographic profile exhibited male and female with age variation from 18-85 years. The results predicted no. of drugs prescribed varies as per age as shown in the bar and pie diagram maximum percentage of drugs is 8.924 % i.e. no. of drugs is 10-15. The results exhibit age variation is directly related to no. of AMA in per counter. The present study emphasises on assessment of use of antibiotics as per guidelines in the hospital environment in order to determine the development of resistance. The study reports out of 250 patients 240 were prescribed with antibiotics and age factor also play an important role in encounter of AMAs. Antibiotic use for patients with diseases of the digestive system needs to be further investigated. The earlier and present investigation visualizes more cross-sectional and in-depth studies are needed in order to control the irrational use of AMAs and to determine the use of AMAs as per guidelines of the healthcare world.