Context: : nitric acid, perenyi's fluid, formic acid, formic-nitric acid, EDTA, decalcifying agent, teeth Aims: To assess the best decalcifying agent among nitric acid, perenyi's fluid, formic acid, formic nitric acid, EDTA for decalcification of teeth Settings and Design: The study was divided into three sets: set I, set II and set III. Each set contained 5 decalcifying solutions with recently extracted permanent 4 teeth i.e. one incisor, one canine, one premolar and one molar in each solution .i.e total 60 teeth. Methods and Material:, Decalcification agents used were NITRIC ACID, PERENIY'S FLUID, FORMIC ACID, FORMIC-NITRIC ACID and EDTA. Radio visiography was used for assessment of decalcification, and its end point. Processing and sectioning with staining of hematoxylin and eosin was done respectively for the specimen The stained sections were observed under a light microscope and grading was done. Statistical analysis used: The results were analysed using descriptive statistics. Discrete (categorical) data were summarized as in proportions and percentages (%) and quantitative data were summarized as Mean ± SD (standard deviation). Inter-rater agreements among the three observers were calculated by using Kappa Statistic. Results: The assessment was done for four parameters such as time of decalcification, ease of sectioning, soft tissue and hard tissue staining and loss of attachment. EDTA was best decalcifying agent while nitric acid was fastest. EDTA though slower decalcifying agent has proven to be the most efficient decalcifying agent among all.