Lesions also known as wounds, lesion/wound infections occur when the skin got cut and the microorganisms like bacteria enter and contaminate and multiplied due topathogenicity. It is therefore necessary for curing infection and preventing damage to the tissues. Infected wound enhances the rate of contamination. Antibiotics are miracle drugs to cure diseases unfortunately, the overuse of antibiotics against harmful pathogens had led to the increase of multidrug resistance in microorganisms.Zinc oxide nanoparticles are inorganic nanoparticles that showed remarkable antibacterial properties against multidrug-resistant bacteria. In this study, zinc oxide nanoparticles are synthesized by roots of mirabilis Jalapa and characterized by UV-Visible spectrometer, SEM, EDX, TEM, XRD, and FTIR respectively to know the morphology, size, and shape. They also act as anti-oxidants and pollution deprivers.