Introduction: Orthodontics nowadays has been progressing greatly in achieving the desired results both clinically and technically through new technologies and methods to accelerate teeth movement and decrease pain. Among various methods, low-level laser therapy was found to be effective by inducing the remodeling process. Aim and objective: To compare and evaluate the effect 810nm and 980nm diode laser on the rate of individual canine retraction Materials and method: A sample of 14 patients involved a split-mouth design study having a laser group and a control group. All cases were aligned till 19*25 ss wire followed by the first premolar extraction. Laser irradiations were given on day 1, 21,42,63. A digital vernier caliper was used to measure the linear distance between the contact point of canine (Distal) and second premolar (Mesial), on Day 1 and day 84 of the study models by a single operator. Result: Significant space reduction was observed from day 1 to day 84 mean difference of 3.35 and 2.28 mm for 810nm and 980nm groups respectively. The laser group 810nm showed a greater reduction than the 980nm diode laser group on day 84 in the laser group. Conclusion: The use of 810nm and 980nm diode laser effectively help in accelerating the orthodontic tooth movement, thereby reducing treatment time. However, 810nm showed greater reduction than 980nm diode laser