The aim of the study was to evaluate and compare the shear bond strength of brackets using moisture insensitive primer (MIP) when exposed to gingival crevicular fluid (GCF), blood and water while bonding. Materials and methods: Sixty non-carious human premolars with sound buccal surface were used in the study. Teeth were divided into 3 groups with 20 teeth in each (n=20) group I (GCF), group II (Blood) and group III (Distilled water). Bonding was done using Transbond MIP and light cure composite in the presence of GCF, blood and water. Shear bond strength (SBS) was assessed using Instron Universal testing machine. The adhesive remnant index (ARI) was calculated using scanning electron microscope (SEM). Comparative statistical analysis was done by unpaired t-test. P value of 0.05 was kept as statistically significant. Results: The shear bond strength value in group I was 11.55±3.94 Mpa (mean±SD), group II was 12.61±4.70 Mpa (mean±SD) and group III was 16.97 Mpa (mean±SD). The unpaired t-test comparison revealed a statistically significant difference with p value of 0.045. ARI scoring showed that in Group 1 more than half of the adhesive resin was present on the bracket base which was comparatively lesser in Group II, and Group III had maximum of the adhesive resin present on the tooth surface