The aim of the study was to compare and evaluate the flexural strength using universal testing machine and surface roughness using surface profilometer of Injection Moulded flexible denture base material and 3D printed Nylon resin. A total number of 60 samples was divided into 2 groups, 30 for each material and further subdivided into 15 each for testing of flexural strength and surface roughness. The average flexural strength of the 3D printed group was higher than that of the injection moulded group which were 0.6720KN and 0.6027KN respectively while the average surface roughness of injection moulded group was higher than that of the 3D printed group which were 2.37793μm and 2.0893μm respectively with a statistical significance value of 0.001. It was concluded that 3D printed nylon resin samples have comparable flexural strength to the injection moulded samples but a lower surface roughness when compared to the injection moulded samples.