At the National Institute of Technology Raipur, Chhattisgarh, India, fourteen horizontal subsurface flow constructed wetlands (HSSF-CWs) were built as part of this research work. The research was done between 2021 and 2022. As a substrate, various soils and gravels were used in this investigation for a constructed wetland system (CWs). The viability of treating wastewater was intentionally created and altered to simulate domestic wastewater. It was investigated using soil beds (entisols, inceptisols, alfisols, and vertisols) with and without plants and gravel beds. The selected macrophytes were Canna indica and Typha latifolia, and the hydraulic retention time (HRT) was 2 days. Every 2 days, the total suspended solids (TSS), biological oxygen demand (BOD5), chemical oxygen demand (COD), total phosphorus (TP), and total kjeldahl nitrogen (TKN) concentrations in the influent and effluent were measured and evaluated to determine how well the wetland units performed in terms of removal efficiency (RE) based on statistical studies. The CWs achieve the maximum TSS removal of 98% for all CWs. As for the BOD5 removal, an RE of 81.7% for planted and 58.9% for unplanted was achieved. As for the COD removal, an RE of 73.6% for planted and 50.4% for unplanted was achieved. As for the TKN, an RE of 62.3% for planted and 43.6% for unplanted was achieved. As for the TP, an RE of 76.2% for planted and 68.3% for unplanted was achieved. RE of TSS 99.6%, BOD5 81.0%, COD 72.4%, TKN 51.8%, and TP 75.3% were achieved for the aggregate base CWs