Introduction:The electrophoresis of plasma protein is a technique to analyse plasma proteins. As per this study the effect of agarose gel concentration in the process of plasma protein electrophoresis has been evaluated focusing in the population of Murshidabad, situated in eastern part of India which was not previously explored. Methods: For conduction of this study a well establish protocol for plasma protein electrophoresis has been used. Tris-glycine buffer and amido black as a stain has been used by this method for protein bands visualisation. The manipulation of the concentration of the agarose gel has been done to observe their effect on protein migration.Agarose gel was prepared in different concentration ranging from 0.6 percent to 1 percent to understand how it impacts on protein migration rates. Results: From our study there is clear correlation between agarose gel concentration and migration rate of plasma protein.Slower protein migration has been observed with higher agarose gel concentration, where as lower concentration of agarose was associated with faster protein movement. Discussion:The relationship which has been observed that is agarose gel concentration and protein migration rates.It can be used in optimization of diagnostic and research condition. Furthermore, this study has been done in Murshidabad, Eastern India. In conclusion, we have tried to put light on the interplay between agarose gel concentration and protein migration in plasma protein electrophoresis