Vinblastine as well as vincristine, two dimeric alkaloids are widely used as anti-cancer medications, are found in the Catharanthus roseus plant. Its cost can be reduced globally by employing an effective method for extracting it from plant matter. Soxhlet Extraction, Solid-liquid Extraction, Supercritical Fluid Extraction (SFE), and Microwave Assisted Extraction are some techniques for extracting chemicals (MAE). The proposed study evaluates the Soxhlet Extraction method which is used to extract the VBL from C. roseus. Extraction was carried out using Soxhlet extraction, in which 10g of vinca roseus plant sample is used with 300mL of ethanol (B.P 78.37 °C) as a solvent in a reflux for 6h at constant temperature 800C [2]. Simple distillation is used to obtain raw Vinblastine from a solution of ethanol at temperature 700C. Cooled extracted samples after dilution Methanol samples were analyzed using an Agilent HPLC 1200 series. The analysis was carried out on an Agilent Zorbax Eclipse XDB-C18 column with a gradient system and a flow rate of 0.2 mL/min. The gradient began with a combination of 70% ammonium acetate (20 mM) and 30% acetonitrile, and the analysis was conducted using a UV detector with a wavelength of 254 nm. [1]. Calibration curve was prepared for quantifying purpose of the actual VBL drug.