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ISSN 2063-5346
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EXTRACTION, IDENTIFICATION, ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF BOSWELLIC ACID USING UV SPECTROPHOTOMETRIC METHOD

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Meera C. Singh, Rukhsana Rub Pinjari, Nikita Bagade, Mayuri Nazirkar, Krishna Sarda
» doi: 10.48047/ecb/2023.12.si7.518

Abstract

Boswellic acid extracted from Boswellia serrata is used as multifunctional drug and one of its uses is in wound healing. Identification of boswellic acid was done by thin layer chromatography and various chemical tests. A simple UV spectroscopic analytical method is developed and validate for the determination of boswellic acid which is not reported yet. The current official method for boswellic acid assay is titrimetry. Result: Boswellic acid has only one double bond in its structure and thus maximum absorption wavelength was found with difficulty at 247 nm, but still in the developed method correlation coefficient (R2) was 0.9992 and Beer's law was followed in the concentrations between 50-250 µg/ml. followed The concentration range in which Beers’s law was is on higher side owing to the fact that boswellic acid has only one double bond, still it is much sensitive than titrimetry. It was discovered that the limits of detection (LOD) and quantification (LOQ) were 17.61515 µg/ml and 53.37923 µg/ml, respectively. The drug's % RSD was found to be less than 2. Conclusion: The analytical approach was found to be specific, precise, linear, accurate, robust, and can be routinely used for the identification and estimation of boswellic acid alone and in formulations in place of official titrimetric method.

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