Introduction: A chronic inflammation of the middle ear and mastoid cavity that lasts for longer than two weeks is referred to as chronic suppurative otitis media (CSOM). A frequent bacteria that causes CSOM is Pseudomonas aeruginosa. Carbapenems are among the most effective antibiotics used against Pseudomonas infections. Resistant to carbapenems is often associated with production of metallo-ß lactamases strains. The detection of the strains that produce MBLs can be useful for ensuring that patients receive the best care possible in an effort to curb and spread resistance. Aim and Objective : To study the molecular profiling of metallo-beta lactamase gene with special reference to blaIMP-1 gene in imipenem resistant Pseudomonas aeruginosa isolates from patients of chronic suppurative otitis media at a tertiary care centre. Material and Methods: The present study was a cross sectional study carried out in the Department of Microbiology and ENT Department for a period of 1 year i.e between June 2022 to June 2023 at Maharajah Institute of Medical Sciences, Vizianagaram. A total of 245 patients clinically suspected cases for CSOM were studied. Swabs taken from discharging ears were sent for Gram’s staining, culture and antibiotic sensitivity test was performed according to the CLSI guidelines 2022. The isolates were further tested for MBL by screening test, by Imipenem – EDTA combined disc test, and MBL E test (Imipenem). The DNA was extracted by using Qiagen DNA Extraction kit, which was further proceeded for the blaIMP-1 gene detection for Pseudomonas aeruginosa by the conventional PCR.