The object of the study was to inspect the potential effect of Gymnemasylvestre (Gs) in Albino rat. Diabetes was induced via a single intra peritoneal injection of alloxan (100 mg/kg). Treatment with Gs extract (50 or 100 mg/kg/day) began two weeks following the administration of alloxan and was continued for five weeks. Diabetic neuropathy is defined as the damage of nerve due to high blood glucose level. High blood glucose level leads to many disease such as renal failure, cataract etc. Alloxan can selectively damage insulin-producing cell. In conclusion amitriptyline leads to no body weight gain, causes increase in grip strength.