Hyperglycemia is the most common metabolic disturbance disorder and has been considered as one of the major mortality causes globaly. Dietary intake of phenolic compounds, flavonoids and antioxidants can offer evidence to promote the restoration of induced hyperglycemia harm. Objective: This study has been done to investigate the impact of ashwagandha roots powder and aqueous extract on rats with increased glucose levels induced by alloxan. Methods: Thirty-six male white albino rats their weights between 150 ±10, were enrolled. For rats‘adaptation, they were fed on basal diet for 7 days. After that, they were classified into two main categories as follows: The first main category (6 rats): Negative control (-ve), normal rats fed on the basal diet. The second one (30 rats): The hyperglycemic rats which were treated by intraperitoneal injection of alloxan 150 mg/kg of their weight. Then the second main group were subdivided into five groups (6 rats each) depending on that: Group (2): Fed on the basal diet as a positive control (+ve). Group (3): Fed on the basal diet containing (2.5% ashwagandha roots powder). Group (4): Fed on the basal diet containing (5% ashwagandha roots powder). Group (5): Fed on the basal diet and given ashwagandha roots extract (2.5%) orally. Group (6): Fed on the basal amount of diet and given ashwagandha roots extract (5%) orally. Serum samples after 28 days of the experiment were assessed for biochemical markers. Results: The obtained results revealed that, treatment by alloxan caused remarkable hyperglycemia and increase levels of cholesterol,triglycerides, VLDL-c, uric acid, urea, LDL-c, creatinine, ALT, AST, and ALP, and reduced HDL-c. Treating rats which were injected by alloxan with the levels from ashwagandha roots powder and extract (2.5 & 5%) showed decreased levels of serum glucose, kidney function, liver function, TC, LDL-c, TG & VLDL-c, and elevated serum HDL-c. Also improved other parameters including BWG,FI and FER. Concoulosion: Rats treated with ashwagandha roots extract (5%) revealed an improvement in the blood glucose, functions of kidney and liver, lipid profile and original histological structure which wasalso restored.