200 samples were collected from patients suffering from different infections and different age groups for both sexes and from different sources distributed on 80 urine samples (Urine), 42 exit samples (Stool), 62 samples (Sputum) and 26 samples from burns and wounds from patients Patients and patients in Al-Diwaniyah Governorate hospitals, whose ages range between 1-60 years, and for a period of five months, from 9/15/2021 to 01/20/2022. Samples were planted on culture media and isolates were diagnosed using traditional methods (culture, microscopic and biochemical). The vitek2 device was also used to confirm the diagnosis of K. pneumoniae isolates, and the confirmation was reinforced by using the molecular diagnosis of the 16 srRNA gene of K. pneumoniae, and the diagnostic results showed a return of 36 K. pneumoniae isolates with a percentage of 22.1%. It was detected phenotypically (using the wooden wheel method) and molecularly the Pcr technique to investigate some genes The results of phenotypic detection of efflux pumps in 18 bacterial isolates showed that they possessed these Acr ABR pumps, and this was confirmed by the molecular detection of efflux pumps, as the results showed that the percentage of the acrA gene was 100%, while the acrB gene was found at 55.5%, and the acrR gene was found in a percentage 100%.