Phytoconstituents are bioactive compounds such as Alkaloids, phytosterols, flavonoids, phenolics, tannins, saponins, steroids, glycosides, terpenes etc. determine the therapeutic value of a medicinal plant either individually or in combination. The current study has performed the phytochemical (phytosterol, alkaloid and flavonoid) isolation of Curcuma aromatica using Thin layer chromatography (TLC) and column chromatography and characterization was done using GS- MS, UV-Vis spectroscopy and FTIR analysis. In the results of TLC analysis, the presence of different phytosterols (lanosterol (0.96 Rf), β-sitosterol (0.88 Rf), stigmasterol (0.83 Rf) and Campesterol (0.28 Rf)), alkaloids (trigonelline- 0.092 Rf), and flavonoids (luteolin (0.57 Rf), quercetin (0.77 Rf) and kaempferol (0.87 Rf ) were identified. During the GC-MS analysis, a total of 11 phytosterols compounds, 7 alkaloids compounds, and 14 flavonoids bioactive compounds were identified in which the Campesterol (phytosterol), lupanine (alkaloid) and Kumatakenin (flavonoid) were identified as the novel compounds. The characterization of the isolated novel compounds was done using UV-Vis spectroscopy and FTIR analysis. The UV-Vis spectroscopy showed different wavelengths for phytosterols, alkaloids and flavonoids (205 nm, 264.59 nm, and 274 nm). Also, during the FTIR analysis, different functional groups of the present bioactive compounds were analyzed.