Context:The high potential variability of chemical composition of the plant material involved in the manufacture of homeopathic mother tinctures (Ǿ) renders quality control and assurance as a significant challenge. In order to assess the quality difference between various brands of homeopathic mother tinctures viz. Arnica montana (AMǾ) and Rhus toxicodendron (RTǾ) were chosen for their evaluation. Aims: The present study aims to check whether they are deficient with required drug strength, developing standardized procedure for evaluating the mother tinctures for its accuracy, sensitivity and reproducibility. This research is an attempt to assess variations in drug strength of different brands of mother tincture. Settings and Design: The Local(L), National(N) and Internationally(I) manufactured AMǾ and RTǾ were standardized by using various physical, chemical and chromatographic parameters and compared with those mentioned in homoeopathic Pharmacopoeia of India (HPI).