The purpose of this study was to determine the antibacterial properties and antibiotic potentials of Benincasa hispida Linn leaves. The study aims to evaluate the antimicrobial activities and determine the inhibition zones for extracts against some bacterial strains and fungi. The antimicrobial activity was investigated in the present study using an ethanolic leaf. The ethnomedicinal plant (an ethnomedicinal herb) was evaluated for its potential to act as an antimicrobial agent against medically significant bacterial and fungus strains. Extracts were tested using agar disc diffusion. The antibacterial and antifungal activities of Benincasa Hispida extracts were evaluated against Gram-positive Staphylococcus aureus and Bacillus Cereus strains, two gram-negative strains, Escherichia Coli & Pseudomonas aeruginosa strains, and two fungal strains Aspergillus Niger & Candida Albicans strains. The zone of inhibitory activity was compared with that of Amphotericin, a standard antifungal medication and Gentamycin, an antibiotic standard. The results indicated that the Bacillus cereus growth was inhibited and that no antifungal effect was seen. The plants were analysed for phytochemicals. Benincasa hispida's microbial activity was due to secondary metabolites. This plant may be used to find bioactive compounds, which could lead to new pharmaceutical research