In the current study, the major- and minor-elemental contents like magnesium, calcium, and iron, zinc, manganese, and copper were determined in the Nerium oleander ayurvedic leaves using the Flame Atomic Absorption Spectrometer (FAAS) technique. The leaf sample was collected from the North-Karnataka region from six different districts, including Bidar, Kalaburgi, Yadgir, Raichur, Bijapur, and Baglkot. The result of the study showed that the concentration level of calcium (Ca) was found to be the highest and that Mg was found to be the second-highest concentration level, which is compared to Fe, Zn, Mn, and Cu. These elemental concentrations were found to be within the permissible limits (FAO/WHO). These elements are crucial for energy metabolism and play a role in preventing certain cancer disorders.