The primary aim of this research is to assess the phytochemical composition, isolation, characterization, and antioxidant potential of extracts from clematis triloba (Ranunculaceae) using various experimental assays. Different solvents, namely aqueous, petroleum ether, chloroform, and ethanol, were employed to extract the medicinal properties of clematis triloba. The study highlights the ethanolic extract of clematis triloba as having the highest antioxidant activity. This investigation emphasizes the significance of medicinal plants, such as clematis triloba, in serving as valuable reservoirs of bioactive compounds for potential use in drug development for human health. The phytochemical screening identified the presence of alkaloids, carbohydrates, sterols, saponins, tannins, flavonoids, phenolic compounds, and terpenoids, underscoring the diverse range of beneficial compounds present in clematis triloba. The Clematis triloba yielded 30.67 ± 0.75 mg gallic acid equivalent/g phenolic contents and 49.36 ± 0.68 mg quercetin equivalent/g flavonoid contents. According to the ethanolic extract different concentrations like 20,40,60,80 and 100 µg/ml were carried out by three methods such as DPPH assay, Hydrogen Peroxide (H2O2) Radical Scavenging Effect and iron (Fe2+) Chelating Activity. As per the antioxidant properties of CTLE of DPPH scavenging showed the highest percentage of activity compared to the other methods of antioxidant. Among the three methods, DPPH showed excellent antioxidant activity compared with other methods and ascorbic acid as a standard. The reductive ability of the extracts increased with a rise in sample concentration. Because of bioactive components, antioxidant activities play an important role in pharmacological functions. Nonetheless, there are several health issues caused by macronutrient deficits that establish an effective approach. This study article explains the current state of herbal therapeutic items across the world, as well as properties ideas for making clematis triloba a better tomorrow in mankind. Therefore, the current study advises against using Clematis triloba as traditional medicines or therapeutic agents for illnesses caused by oxidative stress.