Student interaction in the educational process depends critically on academic motivation. This raised fresh concerns about how to investigate the impact of the online learning environment on students' academic motivation. The current study intends to determine how well online learning settings support the growth of academic motivation in groups of boys and girls pursuing professional master's degrees. The participants are students in the professional master's degree in educational technology's second academic level, and they were split into two experimental groups (one for males and one for girls). The "interactive multimedia" course was studied by the two groups in online learning settings. The academic motivation scale was utilized as a study tool to accomplish the study's objective. The findings indicated that there were no statistically significant differences between groups of boys and girls in the utilization of online learning environments on the growth of academic motivation.