The spleen is the lymphoid organ which is intraperitoneal and situated in left upper quadrant of abdominal cavity between the fundus of the stomach and the diaphragm where it is entirely covered by the inferior thoracic rib cage. The present study was conducted to assess morphometry of spleen in normal adult population as changes in splenic size and volume, splenomegaly is related to many clinical and pathological conditions. Materials & Methods: 124 adult subjects between 25-60 years of age of both genders were included in our study. Dimension of spleen-length, width and thickness and volume were calculated using ultrasonography. Results: Out of 124 subjects,70 were males and 54 were females. The mean length of spleen in males was 10.4 cm and in females was 10.1 cm, width was 6.2 cm and 5.8 cm, thickness was 4.5 cm and 4.2 cm and volume was 112.6 cm3 and 110.4 cm3 in males and females respectively. Conclusion: Our present study concluded that splenic dimensions including length, width, thickness and volume were more in males than females