Vibrio parahaemolyticus (V.parahemolyticus) has become one of the emerging enteric pathogens linked to gastroenteritis, diarrhea, and food poisoning worldwide. Subjects who presented with the clinical features and symptoms of acute diarrhea were included. Of 325 diarrheal samples, majority of the subjects had diarrheagenic Escherichia coli (n = 138), V.parahemolyticus (n = 73) respectively. Thermostable hemolysin and motility was seen in all the isolates of V. parahaemolyticus. Their susceptibility pattern revealed that 8.2%, 26%, 100% were resistant to norfloxacin, ciprofloxacin and ampicillin respectively. Isolates were detected both by molecular and conventional methods using polymerase chain reaction (PCR). Confirmation of V. parahaemolyticus colonies by specific target gene toxR, tdh, trh from the diarrhoeal patients. PCR results showed 100% toxR, 22% tdh and 12.3% trh gene among the isolates. There were limited number of studies found analysing the virulent genes of V.parahemolyticus and its effects on human beings.